Depletion of tyrosyl-DNA phosphodiesterase 1a (MtTdp1a) affects transposon expression in Medicago truncatula

Autor: Sabatini M.E., Donà M., Leonetti P., Minio A., Delledonne M., Carbonera D., Confalonieri M., Giraffa G., Balestrazzi A.
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Journal of integrative plant biology (Online) 58 (2016): 618–622. doi:10.1111/jipb.12457
info:cnr-pdr/source/autori:Sabatini M.E., Donà M., Leonetti P., Minio A., Delledonne M., Carbonera D., Confalonieri M., Giraffa G., Balestrazzi A./titolo:Depletion of tyrosyl-DNA phosphodiesterase 1a (MtTdp1a) affects transposon expression in Medicago truncatula/doi:10.1111%2Fjipb.12457/rivista:Journal of integrative plant biology (Online)/anno:2016/pagina_da:618/pagina_a:622/intervallo_pagine:618–622/volume:58
DOI: 10.1111/jipb.12457
Popis: The role of plant tyrosyl-DNA phosphodiesterase 1? in genome stability is studied using a Medicago truncatula MtTdp1?-depleted line. Lack of MtTdp1? results in a 39% reduction of methylated cytosines as compared to control. RNA-Seq analyses revealed that 11 DNA transposons and 22 retrotransposons were differentially expressed in the Tdp1?-2a line. And among them all DNA transposons (MuDR, hAT, DNA3-11_Mad) and seven retrotransposons (LTR/Gipsy, LTR/Copia, LTR and NonLTR/L1) were down-regulated, while remaining 15 retrotransposons were up-regulated. Results suggest that the occurrence of stress-responsive cis-elements as well as changes in the methylation pattern at the LTR promoters might be responsible for the enhanced retrotransposon transcription.
Databáze: OpenAIRE
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