Deep layers morphology study in human corneas by the use of confocal microscopy
| Autor: | L. Menabuoni (1), A. Malandrini (1), A.Canovetti (1), C.M. Paggini (1), I. Lenzetti (1) Luca Buzzonetti(2), Paola Valente(2) F. Rossi (3), F. Tatini (3), G. Magni (3), R. Pini (3) |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | XXXIV Congress of ESCRS, Copenaghen, 10-14/09/2016 info:cnr-pdr/source/autori:L. Menabuoni (1), A. Malandrini (1), A.Canovetti (1), C.M. Paggini (1), I. Lenzetti (1) Luca Buzzonetti(2), Paola Valente(2) F. Rossi (3), F. Tatini (3), G. Magni (3), R. Pini (3)/congresso_nome:XXXIV Congress of ESCRS/congresso_luogo:Copenaghen/congresso_data:10-14%2F09%2F2016/anno:2016/pagina_da:/pagina_a:/intervallo_pagine |
| Popis: | Title: Deep layers morphology study in Human corneas by the use of confocal microscopy Authors: Luca Menabuoni, Alex Malandrini, Annalisa Canovetti, Ivo Lenzetti, Luca Buzzonetti, Paola Valente, Francesca Tatini, Giada Magni, Francesca Rossi Purpose: to investigate the morphology of predescemetic collagen layers in human corneas, in order to find correlation with age, pathology and to find out new indications to surgical manoeuvres. Setting: Nuovo Opsedale S. Stefano, Prato, Italy; Ospedale Pediatrico Bambino Gesù, Rome, Italy Methods: corneas from patients udergoing trasnplantation and healthy corneas not suitable for transplantation were collected. The samples were grouped into two: one group is from 20 adult subjects aged 59 ± 18 y.o., and the other group is from 15 young subjects, aged 12±5 y.o. The analysis of the deep stroma was performed by confocal microscopy: the corneas were sectioned and incubated with Collagen VI primary antibody, Alexa Fluor-conjugated secondary antibody, and with Phalloidin-Atto 550. Slices were mounted with fluoroshield containing DAPI and visualized using a confocal Leica SP8 scanning microscope. Images were acquired in z-series scans at 0.3 µm intervals. Results: Confocal microscopy analysis of posterior cornea has been performed in order to investigate relevant parameters such as cellular distribution and morphology, and collagen VI characteristics. A predescemetic layer with a higher concentration of collagen I and some samples with a higher concentration of collagen VI was found. High variability due to age and pathology was found. Conclusions: It seems that there is a different distribution of collagen type I and collagen type VI in the deep layers of the cornea, depending on age and pathology. A larger study is needed to improve statistics. Financial Disclosure: all the authors have no financial interests |
| Databáze: | OpenAIRE |
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