Early Detection of Fungal Plant Pathogens by Real-Time Quantitative PCR: The Case of Diplodia sapinea on Pine
| Autor: | Luchi N., Santini A., Salvianti F., Pinzani P. |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Quantitative Real-Time PCR Methods and Protocols, edited by Biassoni R., Raso A., pp. 95–104, 2020 info:cnr-pdr/source/autori:Luchi N., Santini A., Salvianti F., Pinzani P./titolo:Early Detection of Fungal Plant Pathogens by Real-Time Quantitative PCR: The Case of Diplodia sapinea on Pine/titolo_volume:Quantitative Real-Time PCR Methods and Protocols/curatori_volume:Biassoni R., Raso A./editore:/anno:2020 |
| DOI: | 10.1007/978-1-4939-9833-3_8. |
| Popis: | This chapter reports the use of real-time quantitative PCR to detect Diplodia sapinea, a fungal plant pathogen that causes shoot tip dieback and tree mortality on pine trees. This molecular approach represents a reliable and sensitive tool to detect fungal pathogens in DNA extracted from plant tissues and its use can be also recommended to study fungal behavior in host tissues by quantifying fungal growth in the latent phase, when symptoms in the host are not present yet. |
| Databáze: | OpenAIRE |
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