Rapid Chagas diagnosis in clinical settings using a multiparametric assay
| Autor: | Pierimarchi P, Cerni L §, Alarcón de Noya B, Nicotera G, Díaz-Bello Z, Angheben A, Scacciatelli D, Zonfrillo M, Recinelli G, Serafino A*. |
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| Rok vydání: | 2013 |
| Zdroj: | Diagnostic microbiology and infectious disease (2013). info:cnr-pdr/source/autori:Pierimarchi P*, Cerni L §, Alarcón de Noya B, Nicotera G*, Díaz-Bello Z, Angheben A, Scacciatelli D*, Zonfrillo M*, Recinelli G, Serafino A*./titolo:Rapid Chagas diagnosis in clinical settings using a multiparametric assay./doi:/rivista:Diagnostic microbiology and infectious disease/anno:2013/pagina_da:/pagina_a:/intervallo_pagine:/volume |
| Popis: | The development of an immunoassay for detecting circulating antibodies against Trypanosoma cruzi with a good performance appears to be crucial in clinical settings for the management of Chagas disease. Here we propose a new automated ELISA test performed on serum samples using 2 different T. cruzi antigens (a recombinant protein and a T. cruzi whole extract) placed in parallel in separate solid phases. This automated diagnostic tool allows the simultaneous analysis of a large number of sera, by determining the presence of antibodies against both antigens by a single run test, with high sensitivity and specificity. The simultaneous analysis of the reactivity against the 2 antigens in a biparametric modality reduces the percentage of false-negative sera and allows a more accurate diagnosis. Using this multiparametric approach, we propose an effective algorithm for the first step of Chagas diagnosis by performing a single test, with time and cost savings. |
| Databáze: | OpenAIRE |
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