Verbascoside Treatment During In Vitro Maturation Improves The Embryo Development Of Prepubertal Ovine Oocytes

Autor: Dell'Aquila, M E, Ariu, F, Martino, N A, Minervini, F, Cardinali, A, Bogliolo, L
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Zdroj: Reproduction, fertility and development 27 (2014): 243. doi:10.1071/RDv27n1Ab308
info:cnr-pdr/source/autori:Dell'Aquila, M E; Ariu, F; Martino, N A; Minervini, F; Cardinali, A; Bogliolo, L/titolo:Verbascoside Treatment During In Vitro Maturation Improves The Embryo Development Of Prepubertal Ovine Oocytes/doi:10.1071%2FRDv27n1Ab308/rivista:Reproduction, fertility and development/anno:2014/pagina_da:243/pagina_a:/intervallo_pagine:243/volume:27
Popis: Verbascoside (VB), a bioactive polyphenol from olive mill wastewater with known antioxidant activity, was shown to act as a pro-oxidant molecule, by impairing energy/redox status and embryo developmental competence of prepubertal ovine oocytes when added at micromolar concentrations in a continuative 24-h in vitro maturation (IVM) exposure protocol (1). The aim of the present study was to determine whether a lower (nanomolar) VB concentration and a shorter exposure time (2 v. 24h) during IVM may improve the maturation rates of prepubertal ovine oocytes and their subsequent embryonic development in vitro. Cumulus-oocyte complexes derived from the ovaries of slaughtered 1-mo-old prepubertal sheep oocytes underwent IVM in TCM 199 with 10% oestrus sheep serum, 0.1IUmL(-1) of FSH/LH, and 100M cysteamine, in 5% CO2 in air at 38.5°C for 24h. Based on our previous results (Dell'Aquila et al. 2014 Biomed. Res. Int. 2014, 878062), VB was added in the IVM medium at 1.03nM, and 2 incubation times (24 and 2h) were tested. In the 2-h exposure group, after 2h of exposure to VB, oocytes were washed and cultured up to 24h without VB. A group of oocytes were cultured in absence of VB, as controls. Matured oocytes were fertilized with frozen-thawed ram semen in SOF medium for 22h and zygotes were cultured in vitro for 8 days. Metaphase II (MII) cleavage and blastocyst rates were analysed by Chi-squared test. Embryo quality was evaluated by staining and total cell count of the blastocyst and analysis of variance (ANOVA) was applied. Differences were considered to be significant when P0.05). However, this treatment allowed to obtain significantly higher rates of cleaved embryos/MII oocytes (156/196, 80% v. 165/226, 73%; respectively; P
Databáze: OpenAIRE
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