MTERF3, the most conserved member of the mTERF-family, is a modular factor involved in mitochondrial protein synthesis
| Autor: | Roberti M, Bruni F, Loguercio Polosa P, Manzari C, Gadaleta MN, Cantatore P. |
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| Rok vydání: | 2006 |
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| Zdroj: | 1757 (2006): 1199–1206. info:cnr-pdr/source/autori:Roberti M, Bruni F, Loguercio Polosa P, Manzari C, Gadaleta MN, Cantatore P./titolo:MTERF3, the most conserved member of the mTERF-family, is a modular factor involved in mitochondrial protein synthesis/doi:/rivista:/anno:2006/pagina_da:1199/pagina_a:1206/intervallo_pagine:1199–1206/volume:1757 |
| Popis: | The MTERF-family is a wide family of proteins identified in Metazoa and plants which includes the known mitochondrial transcription termination factors. With the aim to shed light on the function of MTERF-family members in Drosophila, we performed the cloning and characterization of D-MTERF3, a component of the most conserved group of this family. D-MTERF3 is a mitochondrial protein of 323 amino acids. Sequence analysis in seven different organisms showed that the protein contains five conserved "mTERF-motifs", three of which include a leucine zipper-like domain. D-MTERF3 knock-down, obtained by RNAi in D.Mel-2 cells, did not affect mitochondrial replication and transcription. On the contrary, it decreased to a variable extent the rate of labelling of about half of the mitochondrial polypeptides, with ND1 being the most affected by D-MTERF3 depletion. These results indicate that D-MTERF3 is involved in mitochondrial translation. This role, likely based on protein-protein interactions, may be exerted either through a direct interaction with the translation machinery or by bridging the mitochondrial transcription and translation apparatus. |
| Databáze: | OpenAIRE |
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