Conditions for calibrConditions for calibration of an isothermal titration calorimeter using chemical reactions
| Autor: | Sgarlata C., Zito V., Arena G. |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
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| Zdroj: | Analytical & bioanalytical chemistry 405 (2013): 1085–1094. doi:10.1007/s00216-012-6565-7 info:cnr-pdr/source/autori:Sgarlata C.; Zito V.; Arena G./titolo:Conditions for calibrConditions for calibration of an isothermal titration calorimeter using chemical reactions/doi:10.1007%2Fs00216-012-6565-7/rivista:Analytical & bioanalytical chemistry (Print)/anno:2013/pagina_da:1085/pagina_a:1094/intervallo_pagine:1085–1094/volume:405 |
| DOI: | 10.1007/s00216-012-6565-7 |
| Popis: | The reaction of protonation of 2-amino-2-(hydroxymethyl)-1,3-propanediol (TRIS) is a suitable one for the calibration of isothermal titration calorimeter (ITC), providing that experimental conditions are appropriately chosen. The conditions and methods for handling experimental data from a nanowatt-ITC are discussed. Also, the binding of Ba2+ to 18-Crown-6 is successfully used to check the accuracy and precision of the chemical calibration performed with TRIS. This latter reaction has the additional advantage that the data can also be used for a check on the determination of the value of a binding constant. The anomaly of the first injection in ITC is analyzed and, by combining calorimetric and spectroscopic measurements, it is shown that it mainly results from a backlash effect of the syringe plunger rather than from a diffusion effect. |
| Databáze: | OpenAIRE |
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