Toxic effects of mycotoxin zearalenone and its derivatives on in vitro maturation of bovine oocytes and 17 beta- estradiol level in mural granulosa cell cultures
| Autor: | Minervini F. 1, Dell?Aquila M.E. 2, Maritato F. 2, Minoia P. 2, Visconti A. 1 |
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| Rok vydání: | 2001 |
| Předmět: | |
| Zdroj: | Toxicology in vitro 15 (2001): 489–495. info:cnr-pdr/source/autori:Minervini F. 1, Dell?Aquila M.E. 2, Maritato F. 2, Minoia P. 2, Visconti A. 1/titolo:Toxic effects of mycotoxin zearalenone and its derivatives on in vitro maturation of bovine oocytes and 17 beta-estradiol level in mural granulosa cell cultures./doi:/rivista:Toxicology in vitro/anno:2001/pagina_da:489/pagina_a:495/intervallo_pagine:489–495/volume:15 |
| Popis: | Moulds parasites of livestock foodstuffs alter the quality of grains by synthetising mycotoxins. Zearalenone (ZEA) and its derivatives (a- and b-zearalenol, zeranol, taleranol and zearalanone) are produced by fungi of the genus Fusarium and, after ingestion via contaminated cereals, may lead to fertility disturbances and other reproductive pathologies. Zearalenone, a-zearalenol and zearalanone were tested, at levels ranging from 0.3 to 30 mg/ml, in order to evaluate the effect on the in vitro maturation (IVM) rate of bovine oocytes and on the formation of 17 b-estradiol in supernatants of mural granulosa cells (GC) cultures. These compounds induced dose-dependent oocyte maturation delay and chromatin abnormalities. Maturation of oocytes to metaphase II (M II) was inhibited in oocytes cultured in the presence of 30 mg/ml ZEA, a-zearalenol or zearalanone, with a significant increase in chromatin abnormalities occurring in the presence of ZEA (P |
| Databáze: | OpenAIRE |
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