Direct Plasma Deposition of Lysozyme-Embedded Bio-Composite Thin Films
| Autor: | Palumbo F., Camporeale G., Yang Y.-W., Wu J.-S., Sardella E., Dilecce G., Calvano C.D., Quintieri L., Caputo L., Baruzzi F., Favia P. |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Plasma processes and polymers (Internet) 12 (2015): 1302–1310. doi:10.1002/ppap.201500039 info:cnr-pdr/source/autori:Palumbo F.; Camporeale G.; Yang Y.-W.; Wu J.-S.; Sardella E.; Dilecce G.; Calvano C.D.; Quintieri L.; Caputo L.; Baruzzi F.; Favia P./titolo:Direct Plasma Deposition of Lysozyme-Embedded Bio-Composite Thin Films/doi:10.1002%2Fppap.201500039/rivista:Plasma processes and polymers (Internet)/anno:2015/pagina_da:1302/pagina_a:1310/intervallo_pagine:1302–1310/volume:12 |
| DOI: | 10.1002/ppap.201500039 |
| Popis: | Bio-composite coatings, consisting of an organic matrix embedding a bioactive molecule, have been deposited by means of atomizer-assisted atmospheric pressure plasma. Ethylene was chosen as the precursor of the matrix, while the atomizer was fed with a water solution of lysozyme. Coatings chemical composition was investigated by XPS, FTIR and MALDI-TOF spectroscopies, and it has been proved that the one-step inclusion of protein domains in the composite coatings is successful and lysozyme chemical structure is only slightly altered. The amount of embedded lysozyme is as high as 14?g/cm2 as evaluated from water release test. Finally, the activity of the plasma-embedded protein is close to that of pure lysozyme as verified against Micrococcus lysodeikticus ATCC 4698 through an agar plate diffusion test. |
| Databáze: | OpenAIRE |
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