Cloning, functional expression and expression studies of the nitrate transporter gene from Chlorella sorokiniana (strain 211-8k)
| Autor: | Koltermann M., Moroni A., Gazzarini S., Nowara D., Tischner R. |
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| Rok vydání: | 2003 |
| Zdroj: | Plant molecular biology 52 (2003): 855–864. info:cnr-pdr/source/autori:Koltermann M., Moroni A., Gazzarini S., Nowara D., Tischner R./titolo:Cloning, functional expression and expression studies of the nitrate transporter gene from Chlorella sorokiniana (strain 211-8k)./doi:/rivista:Plant molecular biology/anno:2003/pagina_da:855/pagina_a:864/intervallo_pagine:855–864/volume:52 |
| Popis: | The nitrate transporter from Chlorella sorokiniana (accession number AY026523) has been cloned by screening a cDNA library based on mRNA isolated after 30 min treatment of Chlorella with 5 mM nitrate and with a RT-PCR product (730 bp) as a probe. The Chlorella sequence has similarity to known nitrate transporters of the NRT2 family (high-affinity nitrate transporters). The cDNA clone was used for functional expression in Xenopus oocytes and a nitrate-dependent current was measured at pH 5.5 but not at pH 7.4. A second algal gene or a second gene product was not needed for functional expression in Xenopus. Inhibitor studies in Chlorella indicated that protein phosphorylation/dephosphorylation is involved in nitrate induction of ChNRT2.1. In addition to nitrate, ChNRT2.1 expression is induced by nitroprusside, a NO donor, and is affected by glucose. |
| Databáze: | OpenAIRE |
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