NRAGE associates with the anti-apoptotic factor Che-1 and regulates its degradation to induce cell death
| Autor: | Di Certo MG, Corbi N, Bruno T, Iezzi S, De Nicola F, Desantis A, Ciotti MT, Mattei E, Floridi A, Fanciulli M, Passananti C. |
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| Rok vydání: | 2007 |
| Zdroj: | Journal of cell science 120 (2007): 1852–1858. info:cnr-pdr/source/autori:Di Certo MG; Corbi N; Bruno T; Iezzi S; De Nicola F; Desantis A; Ciotti MT; Mattei E; Floridi A; Fanciulli M; Passananti C./titolo:NRAGE associates with the anti-apoptotic factor Che-1 and regulates its degradation to induce cell death/doi:/rivista:Journal of cell science/anno:2007/pagina_da:1852/pagina_a:1858/intervallo_pagine:1852–1858/volume:120 |
| Popis: | Neurotrophin receptor-interacting MAGE homolog (NRAGE) has been recently identified as a cell-death inducer, involved in molecular events driving cells through apoptotic networks during neuronal development. Recently, we have focused on the functional role of Che-1, also known as apoptosis-antagonizing transcription factor (AATF), a protein involved in cell cycle control and gene transcription. Increasing evidence suggests that Che-1 is involved in apoptotic signalling in neural tissues. In cortical neurons Che-1 exhibits an anti-apoptotic activity, protecting cells from neuronal damage induced by amyloid beta-peptide. Here, we report that Che-1 interacts with NRAGE and that an EGFP-NRAGE fusion protein inhibits nuclear localization of Che-1, by sequestering it within the cytoplasmic compartment. Furthermore, NRAGE overexpression downregulates endogenous Che-1 by targeting it for proteasome-dependent degradation. Finally, we propose that Che-1 is a functional antagonist of NRAGE, because its overexpression completely reverts NRAGE-induced cell-death. |
| Databáze: | OpenAIRE |
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