Upregulation of neuronal nitric oxide synthase in in vitro stellate astrocytes and in vivo reactive astrocytes after electrically induced status epilepticus
| Autor: | Catania MV1, Giuffrida R2, Seminara G1, 2, Barbagallo G2, Aronica E3, Gorter JA4, Dell'Albani P1, Ravagna A.2, Calabrese V2, Giuffrida-Stella AM2 |
|---|---|
| Rok vydání: | 2003 |
| Předmět: | |
| Zdroj: | Neurochemical research 44(1) (2003): 17–25. info:cnr-pdr/source/autori:Catania MV1, Giuffrida R2, Seminara G1,2, Barbagallo G2, Aronica E3, Gorter JA4,Dell'Albani P1, Ravagna A.2, Calabrese V2, and Giuffrida-Stella AM2/titolo:Upregulation of neuronal nitric oxide synthase in in vitro stellate astrocytes and in vivo reactive astrocytes after electrically induced status epilepticus/doi:/rivista:Neurochemical research/anno:2003/pagina_da:17/pagina_a:25/intervallo_pagine:17–25/volume:44(1) |
| Popis: | Neuronal nitric oxide synthase (nNOS) is a constitutively expressed and calcium-dependent enzyme. Despite predominantly expressed in neurons, nNOS has been also found in astrocytes, although at lower expression levels. We have studied the regulation of nNOS expression in cultured rat astrocytes from cortex and spinal cord by Western blotting and immunocytochemistry. nNOS was not detectable in cultured astrocytes grown in serum-containing medium (SCM), but was highly expressed after serum deprivation. Accordingly, calcium-dependent NOS activity and both intracellular nitrite levels and nitrotyrosine immunoreactivity after glutamate stimulation were higher in serum-deprived astrocytes than in cells grown in SCM. Serum deprivation induced a modification of astrocytes morphology, from flat to stellate. nNOS up-regulation was also observed in reactive astrocytes of rat hippocampi after electrically induced status epilepticus, as demonstrated by double-labeling experiments. Thus, nNOS upregulation occurs in both in vitro stellate and in vivo reactive astrocytes, suggesting a possible involvement of glial nNOS in neurological diseases characterized by reactive gliosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|