Capillary Zone Electrophoresis Study of Cyclodextrin-Lipoic Acid Host-Guest Interaction
| Autor: | Trentin M., Carofiglio T., Fornasier R., Tonellato U. |
|---|---|
| Rok vydání: | 2002 |
| Předmět: | |
| Zdroj: | Electrophoresis (Weinh., Print) 23 (2002): 4117–4122. info:cnr-pdr/source/autori:Trentin M., Carofiglio T., Fornasier R., Tonellato U./titolo:Capillary Zone Electrophoresis Study of Cyclodextrin-Lipoic Acid Host-Guest Interaction/doi:/rivista:Electrophoresis (Weinh., Print)/anno:2002/pagina_da:4117/pagina_a:4122/intervallo_pagine:4117–4122/volume:23 |
| Popis: | Lipoic acid is a naturally occurring compound which is being widely investigated for its therapeutic effects in the treatment or prevention of a variety of diseases associated with oxidative injury, particularly diabetes. The diversity of therapeutic applications of lipoic acid requires an appropriate formulation to control its bioavailability, site- targeting delivery and to overcome its inherent chemical instability. In this regard, cyclodextrins (CDs) are ideally suitable due to their well- documented ability to include in their cavity proper guest molecules and protect them from physical or chemical damages. Lipoic acid forms 1:1 inclusion complexes with bCD as shown in a previous report of an extended investigation that also indicated the suitability of capillary zone electrophoresis (CZE) for the study of such host-guest interactions. In view of these possible applications, we extended the CZE analysis to determine the strength of binding, in a pH 9 phosphate buffer, of lipoic acid with other CD derivatives such as aCD, gCD and the alkylated derivatives of bCD, namely (2-hydroxypropyl)- b-CD (HP bCD), and heptakis (2,3,6-tri-O-methyl)- b-CD (TM bCD). Once established that the easily available bCD is the most suitable receptor for lipoic acid, we set up and here describe a simple and reliable procedure for the quantitative determination of lipoic acid in commercial dietary supplement tablets containing also other active substances and excipients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|