The heparan sulfate proteoglycan Syndecan-1 regulates colon cancer stem cell function via a focal adhesion kinase - Wnt signaling axis'
| Autor: | Katakam Sampath K, Valeria Tria, Sim Wey-Cheng, Yip George W, Stefano Molgora, Theodoris Karnavas, Ileana Zucchi, Rolland Reinbold, Greve Burkhard, Martin Götte |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | 2nd MBE (Matrix Biology Europe) Conference, Athens, Greece, 11-14/06/2016 info:cnr-pdr/source/autori:Katakam Sampath K, Valeria Tria, Sim Wey-Cheng, Yip George W, Stefano Molgora, Theodoris Karnavas, Ileana Zucchi, Rolland Reinbold, Greve Burkhard, Martin Götte/congresso_nome:2nd MBE (Matrix Biology Europe) Conference/congresso_luogo:Athens, Greece/congresso_data:11-14%2F06%2F2016/anno:2016/pagina_da:/pagina_a:/intervallo_pagine |
| Popis: | In colon cancer, downregulation of the transmembrane heparan sulfate proteoglycan syndecan-1(Sdc-1) is associated with increased invasiveness, metastasis, and dedifferentiation. Since Sdc-1 modulates signaling pathways relevant to stem cell function, we tested the hypothesis that it may regulate a tumor-initiating cell phenotype. Sdc-1 small-interfering RNA knockdown in the human colon cancer cell lines Caco2 and HT-29 resulted in an increased side population (SP), enhanced aldehyde dehydrogenase 1 activity, and higher expression of CD133, LGR5, EPCAM, NANOG, SRY (sex-determining region Y)-box 2, KLF2, and TCF4/TCF7L2. Sdc-1 knockdown enhanced sphere formation, cell viability, Matrigel invasiveness, and epithelial-to-mesenchymal transition-related gene expression. |
| Databáze: | OpenAIRE |
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