Prevalence of quinolone resistance mechanisms in Enterobacteriaceae producing acquired AmpC ß-lactamases and/or carbapenemases in Spain
| Autor: | Machuca J., Agüero J., Miró E., Conejo M.D.C., Oteo J., Bou G., González-López J.J., Oliver A., Navarro F., Pascual Á., Martínez-Martínez L. |
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| Rok vydání: | 2017 |
| Předmět: |
antibiotic resistance
enzymology R Factors prevalence R factor beta lactamase AmpC minimum inhibitory concentration bacterial protein quinolone derivative Article beta-Lactam Resistance beta-Lactamases beta lactamase carbapenemase Nalidixic Acid quinolone Enterobacteriaceae Bacterial Proteins ciprofloxacin multidrug resistance plasmid Drug Resistance Multiple Bacterial Humans genetics gene mutation human AmpC beta-lactamases Enterobacteriaceae infection nonhuman isolation and purification bacterium isolate drug effect microbiology Enterobacteriaceae Infections antibiotic sensitivity Spain Genes Bacterial bacterial gene metabolism Fluoroquinolones |
| Zdroj: | ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau instname |
| ISSN: | 0213-005X |
| DOI: | 10.1016/j.eimc.2016.05.006&partnerID=40&md5=95c79fe7f07cb47459a545359abb6d58 |
| Popis: | Background Quinolone resistance in Enterobacteriaceae species has increased over the past few years, and is significantly associated to beta-lactam resistance. The aim of this study was to evaluate the prevalence of chromosomal- and plasmid-mediated quinolone resistance in acquired AmpC ß-lactamase and/or carbapenemase-producing Enterobacteriaceae isolates. Methods The presence of chromosomal- and plasmid-mediated quinolone resistance mechanisms [mutations in the quinolone resistance determining region (QRDR) of gyrA and parC and qnr, aac(6')-Ib-cr and qepA genes] was evaluated in 289 isolates of acquired AmpC ß-lactamase- and/or carbapenemase-producing Enterobacteriaceae collected between February and July 2009 in 35 Spanish hospitals. Results Plasmid mediated quinolone resistance (PMQR) genes were detected in 92 isolates (31.8%), qnr genes were detected in 83 isolates (28.7%), and the aac(6')-Ib-cr gene was detected in 20 isolates (7%). qnrB4 gene was the most prevalent qnr gene detected (20%), associated, in most cases, with DHA-1. Only 14.6% of isolates showed no mutations in gyrA or parC with a ciprofloxacin MIC of 0.5 mg/L or higher, whereas PMQR genes were detected in 90% of such isolates. Conclusion qnrB4 gene was the most prevalent PMQR gene detected, and was significantly associated with acquired AmpC ß-lactamase DHA-1. PMQR determinants in association with other chromosomal-mediated quinolone resistance mechanisms, different to mutations in gyrA and parC (increased energy-dependent efflux, altered lipopolysaccharide or porin loss), could lead to ciprofloxacin MIC values that exceed breakpoints established by the main international committees to define clinical antimicrobial susceptibility breakpoints. © 2016 Elsevier España, S.L.U. y Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica |
| Databáze: | OpenAIRE |
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