MicroRNA-1291 Is Associated With Locoregional Metastases in Patients With Early-Stage Breast Cancer
| Autor: | Escuin D., López-Vilaró L., Bell O., Mora J., Moral A., Pérez J.I., Arqueros C., Ramón y Cajal T., Lerma E., Barnadas A. |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
microRNA 3607
microRNA 6240 non-canonical Wnt signaling microRNA 1291 progesterone receptor Article gene targeting high throughput sequencing breast cancer microRNA 3653 microRNA 3651 middle aged microRNA 182 gene expression profiling microRNA 3535 controlled study human tumor suppressor gene cytokeratin 19 axillary lymph node dissection microRNA lymph node metastasis adult cancer staging protein tyrosine kinase RNA sequencing bioinformatics major clinical study human tissue unclassified drug female real time polymerase chain reaction early cancer microRNA 7641 tumor marker cancer grading histopathology sentinel lymph node metastasis epidermal growth factor receptor 2 Ki 67 antigen microRNA 6516 signal transduction estrogen receptor |
| Zdroj: | Frontiers in Genetics r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau instname |
| ISSN: | 1664-8021 |
| DOI: | 10.3389/fgene.2020.562114&partnerID=40&md5=a774592a8cb6d63785aa5ef57085eba2 |
| Popis: | Evidence that microRNAs (miRNAs) regulate the various steps of metastasis is increasing. Several studies have looked at the miRNA expression profile in primary breast tumors but few have compared primary tumor and sentinel lymph node (SLN) metastasis. We correlated the expression of miRNAs with the SLN status and the outcome of axillary lymph node dissection (ALND) in 60 patients with early breast cancer. We profiled the expression of miRNAs in paired breast tumor samples and SLNs using the NextSeq500 Illumina platform and key findings were validated by qPCR. MultiMiR Bioconductor and Reactome pathways analysis were performed to identify target genes and signaling pathways affected by altered expressed miRNAs. Our results show that nine miRNAs were differentially expressed in tumor tissues (q = 0.05). In tumor samples, a 13.5-fold up-regulation of miR-7641-2 (q < 0.001) and a 2.9-fold down-regulation of miR-1291 (q < 0.001) were associated with tumors with positive SLNs. However, only down-regulation of miR-1291 (q = 0.048) remained significant in paired SLNs samples. Interestingly, a 10.5 up-regulation of miR-1291 in SLNs samples was associated with additional axillary lymph node involvement (q < 0.001). The enrichment analyses showed that canonical and non-canonical WNT pathways and negative regulation of various receptor tyrosine kinases signaling pathways were targets of miR-1291 and supports the role of miR-1291 as a tumor suppressor gene (TSG). Further studies are warranted to investigate the use of miR-1291 as a surrogate biomarker of SLN node metastasis in patients with early-stage breast cancer. © Copyright © 2020 Escuin, López-Vilaró, Bell, Mora, Moral, Pérez, Arqueros, Ramón y Cajal, Lerma and Barnadas. |
| Databáze: | OpenAIRE |
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