MicroRNAs expression associated with aggressive clinicopathological features and poor prognosis in primary cutaneous melanomas
| Autor: | Murria Estal R, de Unamuno Bustos B, Perez Simo G, Simarro Farinos J, Torres Navarro I, Alegre de Miquel V, Ballester Sanchez R, Sabater Marco V, Llavador Ros M, Palanca Suela S, Botella Estrada R |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | MELANOMA RESEARCH r-FIHGUV. Repositorio Institucional de Producción Científica de la Fundación de Investigación del Hospital General de Valencia instname |
| ISSN: | 0960-8931 |
| Popis: | Several studies have focused on identifying microRNAs involved in the pathogenesis of melanoma. However, its association with clinicopathological features has been scarcely addressed. The aim of this study is to identify microRNAs expression profiles related to aggressive clinicopathological and molecular features, and to analyze the association with melanoma survival. A retrospective and observational study was performed in a series of 179 formalin-fixed paraffin embedded primary cutaneous melanomas. First, a screening analysis on a discovery set (n = 22) using miRNA gene chip array (Affymetrix, Santa Clara, California, USA) was performed. Differentially expressed microRNAs were detected employing the software Partek Genomic Suite. Validation of four microRNAs was subsequently performed in the entire series (n = 179) by quantitative real time PCR (qRT-PCR). MicroRNAs expression screening analysis identified 101 microRNAs differentially expressed according to Breslow thickness (=1 mm vs. >1 mm), 79 according to the presence or absence of ulceration, 78 according to mitosis/mm2 ( |
| Databáze: | OpenAIRE |
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