Development of a reliable method based on ultra-performance liquid chromatography coupled to tandem mass spectrometry to measure thiol-associated oxidative stress in whole blood samples

Autor: Escobar J, Sánchez-Illana Á, Kuligowski J, Torres-Cuevas I, Solberg R, Garberg HT, Huun MU, Saugstad OD, Vento M, Cháfer-Pericás C
Rok vydání: 2016
Předmět:
Zdroj: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
r-IIS La Fe. Repositorio Institucional de Producción Científica del Instituto de Investigación Sanitaria La Fe
instname
ISSN: 0731-7085
Popis: The aminothiols are biological compounds with numerous vital functions. One of the most relevant is their role as antioxidant systems. The reduced to oxidized ratios are extremely useful indicators of oxidative stress and cellular redox status. We have validated an ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) method to determine the levels of glutathione, cysteine, homocysteine, and their respective oxidized compounds in whole blood samples. Results showed excellent linearity for all the analytes with correlation coefficients between 0.990 and 0.997, suitable precision with intra-day coefficient of variation
Databáze: OpenAIRE
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