Domestic microwave processing for rapid immunohistochemical diagnosis of bovine rabies

Autor:	Abreu, Camila C., Nakayama, Priscilla A., Nogueira, Clayton I., Mesquita, Leonardo P., Lopes, Priscila F.R., Varaschin, Mary S., Seixas, Josilene do N., Ferreira, Enio, Bezerra Jr, Pedro S.
Rok vydání:	2012
Předmět:	Histopathology Cattle 6 - Ciencias aplicadas::63 - Agricultura. Silvicultura. Zootecnia. Caza. Pesca::636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticos [CDU]
Zdroj:	DIGITUM. Depósito Digital Institucional de la Universidad de Murcia instname
Popis:	The present study describes the use of a microwave processing protocol for the rapid histopathological and immunohistochemical diagnosis of bovine rabies. Immunohistochemistry has been used for rabies diagnosis in formalin-fixed tissue with satisfactory results, although the time to diagnosis is considerably longer than that with direct immunofluorescence. The protocol provided a provisory histopathological rabies diagnosis in approximately three and half hours and the immunohistochemical diagnosis was available after six hours. The protocol achieved 100% correlation with direct immunofluorescence and is a promising method, particularly in situations in which only material in formalin is available for diagnosis or when the refrigeration or transportation of biological material is difficult.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=RECOLECTA___::0f189664744e796a4a53993a84a6ef94 http://hdl.handle.net/10201/54146 Zobrazit plný text záznamu