Ovum Pick up and In Vitro Embryo Production in Boran and Crossbred Dairy Cattle
| Autor: | Jemal, Jeilu, Funga, Asnaku, Ali, Sayid, Dire, Mosisa, Degefa, Tamrat, Yilma, Tefera, Lemma, Alemayehu |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Ethiopian Journal of Agricultural Sciences; Vol. 32 No. 4 (2022); 88-104 |
| ISSN: | 0257-2605 2415-2382 |
| Popis: | This study was conducted to study the potential of Boran (Bos indicus) and Boran * Holstein Friesian Crossbred cows for non-stimulated transvaginal oocyte collections and in vitro embryo production. Follicular aspirations were done using a vacuum pressure pump and Aloka SSD Prosound-2 ultrasound device. 266 aspirations were made in Boran (n=148) and Crossbred (n=118) cattle. The mean number of punctured follicles for combined collections was 7.68 ± 0.4 for both genotypes. The mean number of recovered oocytes for all collections was 2.98 ± 0.2 for both genotypes. The mean number of punctured follicles was 7.27 ± 0.4 and 8.22 ±. 0.44 for Boran and Crossbred cattle, respectively. The mean number of recovered oocytes was 2.65 ± 0.22 and 3.42 ± 0.26 for Boran and Crossbred cattle, respectively. Differences were observed (p0.05) in maturation and cleavage rate of oocytes between the genotypes. A total of 21.5% (14/65) of cleaved oocytes developed to morula stage embryos from both genotypes. Relatively similar patterns were observed in maturation process and embryo development between Boran and Crossbred cattle oocytes. OPU/IVF procedures could be used in local and crossbred heifers as an alternative breeders technology options in animal breeding programs to best exploit the genetic potential of local and exotic breeds. |
| Databáze: | OpenAIRE |
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