| Autor: |
Spelzini, D, Farruggia, B, Guerra, NP, Rua, ML, Pastrana, L |
| Jazyk: |
angličtina |
| Rok vydání: |
2013 |
| Předmět: |
|
| Zdroj: |
African Journal of Biotechnology; Vol 10, No 68 (2011); 15287-15294 |
| ISSN: |
1684-5315 |
| Popis: |
The production of extracellular aspartic peptidase by the fungi Aspergillus niger and Aspergillus awamori was carried out in a shake flask and in stirred tank submerged fermentations using tuna cooked wastewater, an industrial effluent, as nitrogen source for culture medium. In stirred tank fermentation, biomass production of 6 g/l and 2 arbitrary units was obtained. In addition, the partitioning of extracellular aspartic peptidases from cell-free. A. awamori broth culture produced in a stirred tank reactor was carried out in aqueous-two phase systems formed by polyethylene glycol and potassium phosphate. Previous results showed that aspartic peptidases have a high affinity for the PEG-rich phase. A Kp of 4.5 for ATPS PEG 1450-Pi; in ATPS PEG 8000-Pi, Kp value of the range of 2 to 2.5 was obtained. A purification factor 2 was obtained. The method appears to be suitable as a first step for the purification of these proteins from these complex medium.Key words: Tuna cooked wastewater, aspartic peptidases, aqueous two-phase system. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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