Establishment of embryogenic suspension cultures of Pinus radiata Don: research note
| Autor: | Jacoby, A, Van der Westhuizen, AJ, Botha, A-M |
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| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Zdroj: | Southern Forests: a Journal of Forest Science; Vol 194 (2002); 53-58 |
| ISSN: | 2070-2620 2070-2639 |
| Popis: | The development of embryonal suspensor mass (ESM) from immature embryos of Pinus radiata on a solidified growth medium containing 0, 5 mgl -1 benzyladenine, 3, 0 mgl -1 2, 4-dichlorophenoxyacetic acid, 500 mgl -1 casein hydrolysate and 250 mgl -1 L-glutamine was used as inoculum to establish cell suspension cultures. Regular transplants of the ESM established on the solidified medium onto a hormone-free medium ensured sustainable growth and hence a source of ESM. Conditions for optimal growth in liquid media were investigated. The cell suspension turbidity was measured and used as a growth parameter. Optimal growth and normal embryo development were obtained in a liquid medium containing maltose as the carbohydrate source at a concentration of 3% (m/v) and supplemented with 0, 5 mgl -1 2, 4- dichlorophenoxyacetic acid. The results obtained from this study present an opportunity for large-scale propagation of Pinus radiata . Southern African Forestry Journal No.194 2002: 53-58 |
| Databáze: | OpenAIRE |
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