| Autor: |
Olugbuyiro, JAO, Moody, JO, Hamann, MT |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Zdroj: |
African Journal of Biomedical Research; Vol 16, No 1 (2013); 19-24 |
| ISSN: |
1119-5096 |
| Popis: |
The growing problems of tuberculosis have led to the search for new anti-Mtb agents from higher plants. The stem bark of Spondias mombin was evaluated for its in vitro activity against Mycobacterium tuberculosis (H37Rv strain). Bioassay-guided fractionation of the methanol extract was carried out by Vacuum Liquid Chromatography (VLC) on Silica gel (230-400 mesh) and purification was done using HPLC and TLC. In vitro antimycobacterial susceptibility was performed by a fluorometric microplate alamar blue assay (MABA) and percentage mycobacterial inhibition was calculated. The structures of the isolated compounds were established by spectroscopic analysis. The active VLC fraction exhibited 91% inhibition against M. tuberculosis H37Rv at a concentration of 40 μg/mL. The HPLC fraction SMi-15 containing compounds 1 and 2 showed 92.8% inhibition against M. tuberculosis. Two new antimycobacterial phytosterols were isolated from the stem bark of S. mombin and the structureswere identified as mombintane I (1) and mombintane II (2). The stem bark extractives of S. mombin contain antitubercular principles of the class phytosterol and support an important potential of triterpenoids.Keywords: Spondias mombin, Mycobacterium tuberculosis, antimycobacterial, mombintane I, mombintane II |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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