Biodegradation of premium motor spirit (PMS) by lipase from Bacillus thuringiensis and Lysinibacillus sphaericus

Autor: Kareem, S. O., Adegoke, O. O., Balogun, S. A., Afolabi, A. T., Akinde, S. B.
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Nigerian Journal of Biotechnology; Vol 33 (2017); 34-40
ISSN: 0189-1731
Popis: This study reported production of lipase by Bacillus thuringiensis and Lysinibacillus sphaericus. Bacteria isolates were screened on Bushnell-Hass Mineral Salt medium containing 1% PMS for oil degradation. Two potent isolates were identified using 16S rRNA as Bacillus thuringiensis and Lysinibacillus sphaericus. They were cultured for lipase production in a submerged medium. The crude lipases extracted were used for degradation of PMS. Optimum degradation of PMS 44.5% and 37.4% were obtained by lipase from Bacillus thuringiensis and Lysinibacillus sphaericus respectively at pH 7 and 35 in 20 days. This study therefore presented the use of bacterial lipase in degradation of PMS as a simple and effective approach.Keywords: Bacillus spp., biodegradation, lipase
Databáze: OpenAIRE