| Autor: |
El-Sayed, Rehab, Emam, Ahmed, Neamat-Allah, Moustafa, Zeid, Moustafa Abo, Assaf, Raymonde, Baiomy, Amina |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Zdroj: |
Egyptian Journal of Biochemistry and Molecular Biology; Vol 37, No 1-2 (2019); 1-16 |
| ISSN: |
1687-1502 |
| Popis: |
Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5) is a marker for intestinal cancer stem cell and is a target gene of the Wnt/β-catenin signaling pathway, which play an important role in carcinogenesis.In the existing study, evaluation of LGR5, β-catenin and c-Myc mRNA expression by real-time PCR were identified in 40 colon cancer tissue samples in addition to 20 sample from the healthy safety margin of colon cancer as a control.The LGR5, β-catenin and c-Myc mRNA expression levels were detected in HT-29 colon cancer cell line after transfection with siRNA LGR5. Then the cells were devided in to following groups: first group was not transfected and representing the control, the seconed group was treated with Lipofectamine® RNAiMAX transfection reagent only to exclude the toxic effect of Lipofectamine, the third group was treated with siRNA, the fourth group was treated with 5-Flurouracil drug and the fifth group was treated with 5- Flurouracil after siRNA treatement. The viability of the cells in all groups were measured by Vybrant MTT Cell Proliferation Assay Kit.LGR5 expression was elevated in colon cancer tissue samples as compared to that of healthy tissues (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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