| Autor: |
Politeo, Olivera, Carev, Ivana, Burčul, Franko, Jukić, Mila, Ajduković, Petra, Tadijana, Vrdoljak, Miloš, Mladen |
| Jazyk: |
angličtina |
| Rok vydání: |
2010 |
| Předmět: |
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| Popis: |
Alzheimer’s disease (AD) is the most common cause of age-associated memory deficit. It is a degenerative neurological disorder characterized by senile plaques containing amyloid β protein and loss of cholinergic neuromediators in the brain. The most remarkable biochemical change in the brain of AD patients is a reduction of acetylcholine (ACh) levels. Inhibition of acetylcholinesterase (AChE), the enzyme responsible for hydrolysis of ACh, is one of the most established therapeutic approaches in AD treatment. In recent years the free radical hypothesis of aging has brought a better understanding of biochemical events that occur with Alzheimer’s disease. The brain is considered very sensitive to damage caused by oxidative stress for a variety of reasons including, high uptake of oxygen, low glutathione content and high membrane content of polyunsaturated fatty acids. The fact that age is a key risk factor in AD provides considerable support for the free radical hypothesis because effects of the attacks by free radicals, particularly those produced by reactive oxygen species (ROS), can accumulate over the years. Numerous plants and their constituents are reputed in traditional practices of medicine to enhance cognitive function and to alleviate other symptoms of AD, including depression. As a part of our continuing study into discovery of new cholinesterase inhibitors from Croatian plants, the aim of this work was to determine inhibitory potential on acetylcholinesterase as well as antioxidant activity of 39 extracts from 13 selected Croatian plants. AChE inhibitory activities were tested by Ellman method, while antioxidant activities were evaluated using DPPH and FRAP assays. Salix alba L. methanol extract showed the best inhibition of AChE (EC50 =1, 2 g/L), as well as good antioxidant activity correlated with high phenol content determined by Folin - Ciocalteu method. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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