| Autor: |
Batičić, Lara, Detel, Dijana, Buljević, Sunčica, Bedoić, Edvard, Kršek, Antea, Varljen, Jadranka |
| Přispěvatelé: |
Dulić, Morana, Sinčić, Nino, Vrhovac Madunić, Ivana |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Popis: |
Diabetes mellitus and its comorbidities are one of the major health issues worldwide. Diabetes- related complications such as chronic ulcerations are the leading causes of hospitalization of diabetic patients. Dipeptidyl-peptidase IV or CD26 molecule (DPP IV/CD26) is widely known, besides the involvement in a broad range of physiological and pathological processes, for its role in the regulation of glycaemia. DPP IV/CD26 inhibitors are known therapeutics in diabetes. It has been previously shown that DPP IV/CD26 inhibition improves the healing of chronic diabetic ulcers. However, the mechanisms of its actions in the processes and mechanisms of wound healing are unknown. Our hypothesis was that DPP IV/CD26 plays a significant role in the process of cutaneous wound healing in hyperglycaemia through a mechanism of induction enhanced angiogenesis. We aimed to research the process of wound healing in CD26 deficient diabetic mice in order to broaden the knowledge on the role of DPP IV/CD26 in processes of cutaneous reparation and regeneration. A streptozotocin-induced model of diabetes was established in CD26-deficient as well as C57BL/6 (wild-type) mice. Experimental wounds were made on the dorsal region of the mice. Animals were sacrificed in scheduled time periods according to the protocol of wound healing monitoring. Tissue samples were subjected to pathohistological, histomorphometrical, immunohistochemical and immunochemical analyses. Serum samples were analyzed for DPP IV/CD26 activity and concentration of target vascularization factors. The results of this study confirm the hypothesis that DPP IV/CD26 plays an important role in the regulation of glycaemia. Moreover, parameters of wound healing in hyperglycaemia were found to be improved in CD26 deficient mice, indicating that inhibition of DPP IV/CD26 has beneficial effects on the wound healing process in diabetes. Therefore, the significance of DPP IV/CD26 inhibition as a therapeutic strategy for the treatment of diabetes and diabetic wounds is emphasized. DPP IV/CD26 inhibitors are proposed as potential therapeutics in chronic diabetic ulcers. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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