| Popis: |
Selenium is a trace element, required in small amounts for normal functioning of animal organisms. Even though essential, if present in concentration higher than necessary it becomes toxic and results in development of serious conditions. Despite this fact, researches concerning its toxicity effects on soil animals are scarce. The aim of this study was to investigate effects of two forms of selenium, selenite and selenate, on the immunological system of the earthworm Eisenia andrei, using coelomocytes, effector cells of its immune- system. To assess earthworm’s ability of defence against pathogens after exposure to selenium, phagocytosis assay was done. Additionally, ROS and NO levels were measured. Results showed that selenium does not impair earthworm’s capability of phagocytosis or cause significant changes in NO levels. Moreover, at concentration of 5 µg/cm2 selenate causes decrease in ROS content in coelomocytes. |
