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Transmissible Spongiform Encephalopathy's (TSE) are the group of fatal disease, which is known for more than 100 years. They are contagious inside the species and between different species. They are caused by prions, protein particles, and 250 amino acids long. The most famous TSE in sheep population is scrapie which is a fatal, degenerative disease that affects the nervous systems of sheep and goats. Genetic predisposition for diesis was determined and defined by prion protein gene polymorphism. Main task of this work is estimate PrP gene polymorphism in sheep population of Bosnia and Herzegovina (privorski strain). On the basis of result genetic risk would be estimated, trough definition of different genotypes presented in population. This would be helpful for increasing selection with excluding of undesired genotypes, preventing and decreasing of frequency of disease. This work is based on blood samples taking from sheep population in Bosnia and Herzegovina, DNA extraction from fool blood, PCR amplification, sequencing and defining of genotypes. Part of PrP gene, exon 3 was sequenced for 53 sheep belonging to Bosnian autochthonous breed (pramenka). Analyses has shown that alleles ARQ, ARR and AHQ were predominant with frequencies 45.28%, 36.79% and 16.98% respectively, while VRQ 0.94% allele were rare. Synonymous mutations on codons 231 (R) and codon 237 (L) has shown. The polymorphism of PrP gene in Bosnian sheep breed pramenka was not critical with respect to scrapie susceptibility and with some efforts number of "favorable" genotypes can be increased. |
