| Autor: |
Tišljar, Marina, Grabarević, Željko, Artuković, Branka, Džaja, Petar, Foršek, Jadranka, Barić Rafaj, Renata |
| Přispěvatelé: |
Fujisaki, K., Memon, M. A., Gurbuz, Y. |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
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| Popis: |
Experiment A. Chronic toxicity. 140 one day-old male broiler chicks divided in four groups. Every other day: intraperitoneal (ip) L-NAME (LN, 10 mg/kg BW) ; L-arginine (LA, 100 mg/kg BW), L-arginine and a L-NAME combination (100 and 10 mg/kg BW, respectively), and physiological saline (0.90% w/v ; 0.5 mL/kg BW). Seven birds were euthanized weekly during their first five weeks of life. Experiment B. Subchronic toxicity. Equal doses of the same substances: ip every eight hours into 38 day- old birds over 36 hours. Experiment C. Acute toxicity. Thirty day-old birds: one ip dose of various concentrations of L-NAME (50, 100 and 150 mg/kg BW) ; L-arginine (100 mg/kg BW) and saline (0.5 ml/kg BW). All the birds were euthanized after six hours. The end of experiment A: the gross finding of ascites- pulmonary hypertension syndrome (PHS) confirmed vasoconstrictory effect of L-NAME in five birds (LN). Toward the end of experiment A ; in experiment C: histopathological findings (myocardial/pulmonary oedema /LN/ ; congestion/ haemorrhages /LA/) the most prominent (higher LN-doses /100 and 150 mg/kg BW/). Irreversible myocardiolysis and hepatocellulolysis: confirmed in all three experiments (LN). Focal myocardial degeneration solely: in the L- NAME/L-arginine simultaneously treated group. Haematological and blood chemistry values ; stress index value ; and relative organ weights agreed with well-known literature data on PHS. Experiments A and C. The severity of LN- provoked hypoxic changes and plentiful haemorrhages (LA) - dose and time dependent. Mild changes in L-NAME/L-arginine group confirmed a protective role of LA. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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