| Popis: |
Asperigllus flavus is a well-known ubiquitous fungus, which in certain conditions can produce secondary metabolites, namely, mycotoxins, that are hazardous for animal and human health. Mycotoxins are able to contaminate different feed and food commodities and due to their direct influence on human health, this issue is raising the attention of the European Community. The awareness that different chemicals, largely used in agriculture to control pests, could be hazardous for both the environment and human health, led to large limitation of their use. In particular, since 2014, the EC has banned about 50% of chemicals used in agriculture. Furthermore, presently the EC is encouraging the researchers to investigate more environmental friendly “green” approaches and ecocompatible tools a in control of plant fungal diseases, for preventive and/or detoxification strategies. Thus, the necessity of environmentally friendly alternatives, able to control aflatoxin synthesis has increased. One of the most promising biocontrol agents is Trametes versicolor, an edible and non-toxic Basidiomycete, considered “healing mushroom” for its bioactivity towards some pathologies. The exo-polysaccharide was extracted, purified, its structure was characterized and the product was named Tramesan. Our work demonstrates that oligosaccharides derived from polysaccharide Tramesan®, have the ability to counteract the mycotoxin synthesis in Aspergillus flavus. The inhibiting effect on aflatoxin synthesis of these compounds was evaluated. The aim of the study was to determine the smallest part of the polysaccharide of the Tramesan, active in the inhibition of aflatoxin B1 synthesis. The results have shown that the heptasaccharides have the most inhibiting effect on aflatoxin synthesis. In conclusion, a biocontrol agent from T. versicolor could be considered new eco-compatible tools for mycotoxins control, in line with EU directives. |
