| Popis: |
Fungi can degrade lignocellulolytic materials by secreting complex enzyme cocktails composed of cellulolytic and lignolytic enzymes. In this work, seven fungal species (Mucor indicus DSM 2185, Paecilomyces variotii CBS 372.70, Myceliophthora thermophila CBS 663.74, Thielavia terrestris CBS 456.75, Botryosphaeria dothidea JCM 2738, Fusarium oxysporum f.sp. langenariae JCM 8293 and Fusarium verticillioides JCM 23107) grown in four different media were screened for lignocellulose degrading activity. Enzymatic activity assays for the hydrolase’s xylanase, mannanase, arabinase, endo- and exo-glucanase, and pectinase, as well as for the oxidoreductase’s laccase, lignin peroxidase and cellobiose dehydrogenase were employed. From the seven tested fungi, Fusarium oxysporum f.sp. langenariae JCM 8293 and Fusarium verticillioides JCM 23107 were selected as the best producers of lignocellulolytic enzymes, and a more detailed investigation was performed. Sequential solid-state and submerged cultivation of F. oxysporum and F. verticilliodes was used to induce an increased production of lignocellulolytic enzymes. After six days of cultivation the fermentation broth was centrifuged, and the obtained supernatant was partially purified and concentrated. Enzymatic activities in both extracts were determined and the mold F. verticillioides showed higher xylanase activities than F. oxysporum, while the latter showed higher glucanase activities. It can be concluded that both strains can be used as producers of enzyme cocktails for the degradation of lignocellulose containing agro-waste. |
