| Autor: |
Mahovlić, Vesna, Audy-Jurković, Silvana, Ovanin- Rakić, Ana, Škopljanac-Mačina, Lada, Barišić, Ana, Ilić-Forko, Jadranka, Škrablin-Kućić, Snježana, Božikov, Jadranka |
| Přispěvatelé: |
Jonjić, Nives, Kardum.Skelin, Ika |
| Jazyk: |
angličtina |
| Rok vydání: |
2005 |
| Předmět: |
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| Popis: |
Aim of the study. To determine morphometric variables and nuclear DNA content in endometrial cytologic samples by use of image analysis. Patients and Methods. Feulgen method of staining (Merck, Germany) was used on May- Grünwald-Giemsa stained archival aspiration cytology endometrium specimens with the following histopathologic diagnoses: proliferation, hyperplasia simplex, hyperplasia complex, hyperplasia complex atypica, and adenocarcinoma endometrii grade I (n=5 each). Image analysis was performed by use of the Sform (Vamstec, Zagreb) software. The image (x100 oil) was transferred from a microscope (Olympus BHS) via CCD TV color camera (Sony) to a PC by digitalized image. Integrated optical density (IOD) as a measure of nuclear DNA content was calculated for each nucleus (n$100). Lymphocytes (n$20) were used as internal control, along with the factor of correction. The morphometric parameters of nuclear area, nuclear perimeter, and factors of nuclear regularity and nuclear ellipticity were analyzed. Statistical analysis was done by use of Statistica ver. 6.0. Results. Multivariate analysis (ANOVA) yielded significant differences (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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