Genetic diversity in European perennial ryegrass cultivars investigated with RAPD markers

Autor: Bolarić, Snježana, Barth, Susanne, Melchinger, E. Albrecht, Posselt, K. Ulrich
Jazyk: angličtina
Rok vydání: 2005
Předmět:
Popis: Perennial ryegrass (Lolium perenne L.) is the most important grass species for temperate grassland agriculture. The genetic relationship and distance among cultivars is largely unknown but of great interest for breeding programmes. The objectives of this study were to (i) investigate the molecular variation and structure of population cultivars, (ii) describe the relationship among cultivars in terms of the modified Rogers' distance, and (iii) determine the minimum sample size required for characterization of cultivars of L. perenne using random amplified polymorphic DNA (RAPD) markers. A total of 22 ryegrass cultivars, mainly of European origin, were investigated with RAPD markers. The minimum sample size required for the characterization of cultivars was about 20 individuals per population. An analysis of molecular variance (amova) revealed a much larger genetic variation within cultivars (66%) than between them (34%).
Databáze: OpenAIRE