Determination of the optimal conditions for the biodegradation of polyvinyl chloride by the yeast Candida parapsilosis using a full factorial plan

Autor: Kuštro, Marija, Tavra, Dubravka, Bule, Kristina, Prevarić, Viktorija, Cvetnić, Matija, Ukić, Šime, Bolanča, Tomislav, Miloloža, Martina, Markić, Marinko, Furač, Lidija, Kučić Grgić, Dajana
Přispěvatelé: Kassal, Petar, Meštrović, Ernest, Namjesnik, Danijel, Ribić, Rosana, Šekutor, Marina, Tomišić, Vladislav, Usenik, Andrea
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Popis: The introduction of plastic waste into the components of the environment is increasing. We find plastic particles smaller than 5 mm in the environment, which we call microplastics (MP). MP has been attracting increasing attention for years and is considered a new pollutant in the environment. During the production of plastic products, various additives are added to improve their chemical and physical properties.1 As a new pollutant, MP needs to be removed from the environment, so recently research has been conducted to find possible solutions for its removal. A combination of physical, chemical and biological processes can enhance the removal of MP from the environment. Microorganisms have a good potential for MP biodegradation which is an environmentally acceptable process.2 In this research, the optimal conditions for biodegradation were investigated according to the design of a full-factorial plan. For this purpose, the study was conducted at three different optical densities (OD) (0.1, 0.5 and 1.0), pH values (3, 5 and 7) and revolutions per minute (120, 160 and 200). During the 30 days of the biodegradation experiment, changes in OD, pH values and the total number of grown yeast colonies (CFU) were monitored. For a better insight into the biodegradation process, TOC analysis, HPLC/MS analysis of filtrate, and FTIR-ATR analysis of MP were carried out. The toxicity of the filtrate was determined using the bacterium Vibrio fischeri. The experimental results were processed by statistical analysis of variance (ANOVA) using a full factorial experimental design. This study showed that the optimal conditions for PVC biodegradation are the rpm, at low values (120 rpm), at a higher OD (1.0) and an intermediate pH (5).
Databáze: OpenAIRE