| Autor: |
Čavka, Ana, Novak, Sanja, Mihaljević, Zrinka, Grizelj, Ivana, Ćosić, Anita, Lončarić, Zdenko, Popović, Brigita, Drenjančević, Ines |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
|
| Popis: |
Objective:Trace elements zinc (Zn) and selenium (Se) are important components of antioxidative enzymes. The aim of this study was to determine if reduced dietary Zn and Se levels affect vascular function by increasing oxidative stress. Materials and Methods: Thirty three male Sprague Dawley rats were fed with 4 types of custom made rat chow (Faculty of Agriculture University of Osijek, Croatia) from weaning for 10 weeks: a) high Zn(70.81 mg/kg)-high Se (0.363 mg/kg) (N=5) ; b) high Zn(70.81 mg/kg)- low Se(0.043 mg/kg) group (N=10) ; c) low Zn(30.16 mg/kg)-high Se (0.363 mg/kg) group (N=10) and d) low Zn(28.56 mg/kg)-low Se(0.030 mg/kg) group (N=8). Prior to decapitation, rats were anesthetized with 75 mg/kg ketamine+2.5 mg/kg midazolam. Dose responses to ACh (10-9- 10-5 M) and response to reduced pO2 (bath gas mixture containing N2 95%, CO2 5%) were tested in noradrenaline-precontracted aortic rings in the absence/presence of the NOS inhibitor L- NAME, COX-1, 2 inhibitor indomethacin (INDO) and superoxide scavenger Tempol in tissue bath. To test differences among groups Two-way ANOVA or One-way ANOVA was used when appropriate (SigmaPlot v11.2, Systat Software, Chicago, USA). Results:ACh induced relaxation (AchIR) was reduced in low Zn-low Se group compared to other groups (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
