Separation of bioactive compounds from tobacco waste using the sequence of supercritical CO2 extraction and subcritical water extraction

Autor: Banožić, Marija, Gagić, Tanja, Škerget, Mojca, Čolnik, Maja, Jokić, Stela
Přispěvatelé: Pintar, Albin, Cerc Korošec, Romana, Lisjak, Darja, Lozinšek, Matic, Novak Pintarič, Zorka, Valant, Matjaž, Zupančič, Silvo
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Popis: The food and plant industry generate large amounts of wastes or by-products annually around the world from a variety of sources. Improper management and illegal dumping of waste, particularly hazardous and toxic waste, possess increasing threats to the environment and human health. Tobacco waste is a by-products produced in large quantities during processing of tobacco and in most cases, represents an inefficient use of valuable compounds. Tobacco waste (type: scrap) in this study was obtained from “Fabrika Duhana Sarajevo” from Bosnia and Herzegovina. A series of operational parameters of supercritical CO2 (SC- CO2) extraction of tobacco waste (pressure: 100– 300 bar, temperature: 40–80 °C), were investigated in a laboratory scale apparatus in order to get extracts rich in nicotine. The CO2 mass flow rate was kept constant during the process. From reused tobacco waste (after SC-CO2 extraction), subcritical water extraction (SWE) under selected conditions (solvent/solid ratio: 28 mL/g, temperature: 150 ˚C, time: 23 min) was sequentially performed to obtain the extracts rich in phenolic compounds. The content of bioactive compounds was determined using High Performance Liquid Chromatography (HPLC) and by gas chromatography (GC-MS) Obtained extracts of tobacco waste after SC-CO2 extraction were characterized by high levels of nicotine, and after SWE extracts were characterized by a high content of phenolic compounds, considerable amounts of nicotinic acid and nicotinamide and reduced content of nicotine. Also, presence of 5- HMF, furfural and 5-MF was detected in SWE extracts.
Databáze: OpenAIRE