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Metal content in the samples of freshwater sponge Eunapius subterraneus which is an endemic species of karstic underground in Central Croatia was determined by inductively coupled plasma atomic emission spectrometry (ICP–OES). Additionally, metal content was determined in the samples of water and sediment collected at the same sampling sites. Analytical procedure of sample preparation included acid digestion in open vessel. The accuracy of the applied methods tested by measurement of certified reference samples of water and sediment comprised 5–10% of declared metal concentrations. It was established that metal content (Al, Ba, Ca, Cd, Cu, Fe, Mg, Mn, Ni, Pb, Sr, and Zn) measured in sponge tissue, water and sediment samples is related to sampling site. Influence of anthropogenic pollution through sewage deposition was observed in slightly higher content of Al, Cu, and Zn in water, and also Cu, Pb and Zn in sediment sample. Analysis of two morphologically different sponge samples showed different abundance of Cu, Zn, Pb and Cd. |
