| Autor: |
Butorac, Dražan, Grdić Rajković, Marija, Ćelap, Ivana, Kačkov, Sanja, Miletić, Tomislav, Žanić Grubišić, Tihana |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
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| Popis: |
Paraoxonase 1 (PON1) is a enzyme whic is associated with high density lipoprotein (HDL) in serum. PON1 posses paraoxonase, arylesterase and lactonase activity and hidrolyze different kindes of supstrate. Also, PON1 have antioxidant/antiatherogenic activity, and it removes carcinogenic radicals of lipid peroxidation in human body. It was established earlyer that patients with premalignant lesions of cervix have increased lipid peroxidation and changes in antioxidative system, which points importance of oxidative stress in early phase of carcinogenesis. AIM: We carried out this study in order to determine PON1 arylesterase activity in patients with premalignant lesions of the cervix. METHODS: Study included 65 women [38 (32-46) years] with a biopsy confirmed diagnosis of cervical intraepitelial neoplasia (CIN), 18 patients had CIN1, 23 had CIN2 and 24 had CIN3. 109 healty volonteers [37 (29-42) years] without any cervical or other disease were enroled in the study as a control group. Concentration of HDL cholesterole and apolipoprotein AI (apoAI) were determined by commercially available reagents. PON1 arylesterase activity was assayed spectrophotometrically with phenilacetate as a substrate. We standardized PON1 arylesterase activity according to the concentration of HDL- cholesterol RESULTS: PON1 arylesterase activity was significantly reduced in group of patients (53 ± 20 kU/L vs 75 ± 18 kU/L ; P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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