| Popis: |
The aim of this study was to compare AMF abundance and diversity between cultivated grapevine and its wild relative Vitis vinifera subsp. sylvestris. AMF abundance and diversity were explored in 21 wild grapevine plants in the wide area of Neretva River in southwestern Bosnia and Herzegovina and in eight production vineyards in the neighbouring southern Croatia, whole area with (sub) Mediterranean climate. AMF colonisation was 90-100% in the wild grapevine versus 40%-50% in conventionally cultivated grapevine or up to 70% in organic vineyards, on V. berlandieri×V. rupestris rootstock. Vesicular and arbuscular colonisation of wild grapevine reached 54% and 69% respectively, compared to 21% and 25% in vineyards. AMF spore abundance was similar for both relatives, ~100-1300 per 100 g of soil. In the wild grapevine the majority of the AMF sequences were similar to unidentified Glomus species, with only G. iranicum iden-tified. In vineyards G. intraradices/G. fasciculatum, G. sinuosum/G. coremioides, G. vis-cosum, G.indicum/G. iranicum, and a group of unidentified sequences were found. The wild grapevine was determined as highly AMF receptive with colonisation levels well above the levels found for cultivated grapevine in the same region. Data on AMF diversity in wild grapevine remain preliminary at this stage, with only G. iranicum identified. Inter-estingly, some unidentified Glomus species both from wild grapevine and from organic vineyards grouped together and further wider sets of sequences will show whether these AMF sequences could be characteristic for grapevine, as to date they have not been rec-orded for other plant species. |
