| Autor: |
Kovačević, Goran, Gregorović, Gordana, Horvatin, Karlo, Krstanac, Željko, Domijan, Ana-Marija, Želježić, Davor, Peraica, Maja, Kalafatić, Mirjana |
| Přispěvatelé: |
Kniewald, Jasna |
| Jazyk: |
angličtina |
| Rok vydání: |
2006 |
| Předmět: |
|
| Popis: |
Fumonisins are a family of mycotoxins produced by Fusarium sp. (mostly F. moniliforme and F. proliferatum). Exposure to fumonisins is associated with a variety of adverse health effects in domestic and experimental animals. Among more than 10 types of fumonisins, the most prevalent and the most toxic is fumonisin B1 (FB1). Apoptosis is a programmed cell death characterized by a cytoplasmic shrinkage and condensation of chromatin in nucleus. The aim of this study was to assess whether low doses of FB1 induce apoptosis in rat liver at different lag time after treatment. Adult, male Wister rats (6 per group) were given single oral dose of FB1 (5, 50, and 500 μ g/kg b.w. dissolved in sterile saline) and sacrificed 4, 24, and 48 hours afterwards. Control animals were given sterile saline or Fe-NTA. Whole liver was fixed in formalin, routinely processed, and embedded with paraplast. Sections of 4 µ ; ; ; m were stained with hematoxyllin and eosin (HE). Number of hepatocytes undergoing apoptosis was determined by counting 500 cells (computer software Lucia G 4.81) in 25-50 randomly selected microscopic fields of vision (microscope Nikon Eclipse E600). Data were presented as mean with standard deviation, median, minimum and maximum values, and geometrical mean. Possible differences in treatment and control groups were evaluated using t-test. Probability values of P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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