| Autor: |
Peruč, Dolores, Tićac, Brigita, Begić, Gabrijela, Malenica Staver, Mladenka, Abram, Maja, Gobin, Ivana |
| Přispěvatelé: |
Abram, Maja, Bielen, Ana, Kifer, Domagoj, Maravić Vlahoviček, Gordana, Šegvić Klarić, Maja |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
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| Popis: |
Nontuberculous mycobacteria (NTM) are natural inhabitants of natural waters, drinking water distribution systems and soils. Because of their ubiquitous distribution, people are surrounded by these opportunistic pathogens. The hydrophobicity of NTM greatly contributes to aerosolization, favors surface adhesion, biofilm formation, as well as, disinfectant and antibiotic resistance. Therefore, there is a need to extract substances with antituberculous activity from natural sources such as plants. Antimycobacterial activity of the essential oil (EO) of Juniperus communis (J. communis) and Thuja occidentalis (T. occidentalis) against Mycobacterium avium ssp. avium (serotype 2) ATCC 25291 (M. avium), Mycobacterium intracellulare ATCC 13950 (M. intracellulare) and Mycobacterium gordonae ATCC 14470 (M. gordonae) strains was investigated. To determine the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) the broth microdilution method in Middlebrook 7H9 broth was used. The EO combinations were tested by checkerboard synergy method. The initial concentration of J. communis and T. occidentalis EO in synergy method was 3.2 mg/ml and 25 mg/ml respectively. The MICs and MBCs for J. communis EO were 1.6 mg/ml against all of the tested strains. For T. occidentalis EO MIC and MBC were 6.4 mg/ml against M. avium and M. intracellulare and 12.8 mg/ml against M. gordonae. The lowest concentrations of EOs in checkerboard synergy method with effect on different Mycobacteria were for J. communis EO four times lower (0.1 mg/ml) and for T. occidentalis EO twice lower (1.56 mg/ml and 3.125 mg/ml) than MIC and MBC. To conclude, the investigated EOs showed a synergistic antimycobacterial effect in subinhibitory concentrations which opens the possibility of its use as an alternative natural disinfectant. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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