| Popis: |
The liver and muscle tissue of the common two-banded seabream, Diplodus vulgaris from the North Adriatic have been analysed. Total lipids were extracted from the samples according to Folch et al. [1]. The total lipid content amounted to 1, 1 +/- 0, 3 % in the edible muscle tissue and 4, 5 +/- 1, 0 % in the liver. Neutral (triacylglycerols) and polar (phosphatidylinositol, phosphatidylserine, phosphatidylcholine, phosphatidylethanolamine) lipid fractions were separated from the total lipid extract by thin-layer chromatography [2]. The fatty acid compositions of neutral and polar lipid fractions were determined by gas chromatography of the corresponding methyl esters. There have been identified 16 different fatty acids. The major constituents of the total fatty acids in both analyzed samples were saturates: palmitic (20, 8 - 43, 4 %) and stearic acid (6, 3 - 33, 7 %) ; monoenes: oleic (10, 0 - 33, 6 %) and palmitoleic acid (2, 1 - 11, 0 %) ; polyunsaturates: arachidonic acid (1, 5 - 8, 6 %), docosatrienoic acid (0, 8 - 11, 6 %), eicosapentaenoic (1, 1 - 5, 5 %) and docosahexaenoic acid (1, 5 - 9, 9 %). Statistically significant diference in the relative ratios of the eicosapentaenoic acid was found in phosphatidylethanolamine when comparing muscle and liver tissue (p |
