| Popis: |
Investigations were carried out on trout larvae (Salmothymus obtrusirostris, Heckel 185) age of 10-56 days caught from Vrljika river (Croatia). Larvae were taken every two days and fixed in 10% buffered formalin. The fixed material was embedded in paraffin and serially longitudinally cut in the 10 micrometers thick sections and were stained with hematoxylin and eosin (HE), Toidin blue (TB) specific staining, Periodic Acid-Shiff reaction (PAS) and Alcian blue-specific (pH=2.5) (AB) method to show the tissue structure. Development of the larvae were analyzed, primarily the development of muscle tissue. The number of myosepts on the 10 larvae were measured in the period of 10-18, 20-28, 30-38 and 40-48 and 50-56 days. In these periods average number of myosepts increased and it was on 10-18 days 42.82, 22-30 days 49.78, 30-38 days 53.57, 40-48 days 57.04 and 50-56 days 60.33 myosepts. |
