| Autor: |
Priymenko, N, Tache, S, Steghens, JP, Milkovic, L, Borovic-Sunjic, S, Zarkovic, N, Gaultier, N, Naud, N, Pierre, F, Gueraud, F |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
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| Popis: |
The end products of polyunsaturated fatty acid (PUFA) peroxidation, such as malondialdehyde (MDA), 4-hydroxynonenal (HNE) and isoprostanes (8-iso-PGF2α) are widely used as systemic lipid oxidation/oxidative stress biomarkers. However, some of those compounds have also a dietary origin. Thus, replacing dietary saturated fat by PUFAs would improve health but could also increase the formation of such compounds, especially in the case of a pro-oxidant/anti-oxidant imbalanced diet. Hence, possible impact of dietary fatty acids and pro-oxidant compounds was studied in rats treated by 2x2 protocol based diets allowing comparison of the effects of heme iron vs ferric citrate and of ω-6 vs ω-3 rich oil on the level lipid peroxidation/oxidative stress biomarkers. The results obtained have shown that MDA and the major urinary metabolite of HNE (the mercapturic acid of dihydroxy-nonane, DHN-MA) were highly dependent on the dietary factors tested, while 8-iso-PGF2α was modestly but significantly affected. Intestinal inflammation and the tissue lipid composition were checked in parallel and could only explain the differences we observed in a limited extend. Thus, the differences in biomarkers were attributed to the formation of lipid oxidation compounds in food or during digestion, their intestinal absorption and their excretion into urine. Moreover, fecal extracts from the rats fed on the heme iron or fish oil diets were highly toxic for immortalized mouse colon cells. Such toxicity can eventually lead to promotion of colorectal carcinogenesis supporting the epidemiological findings between red meat intake and colorectal cancer risk. Therefore, the analysis of these biomarkers of lipid peroxidation/oxidative stress in urine should be used with caution in case dietary factors are not well controlled, while control of their possible dietary intake is needed also because of their pro-inflammatory, toxic and even co-carcinogenic effects. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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