| Autor: |
Muhvic Urek, Miranda, Borčić, Josipa, Uhač, Ivone, Pezelj-Ribaric, Sonja, Ferreri, Silvio, Bralic, Marina, Tomac, Jelena, Kovač, Zoran, Delić, Zdravko |
| Jazyk: |
angličtina |
| Rok vydání: |
2003 |
| Předmět: |
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| Popis: |
Radiation-induced damage of salivary glands is seen soon after the initiation of treatment and at relatively low doses. The mechanism of this is not understood yet. Objective: The purposes of this study were to examine the apoptosis and proliferartion of parenchymal cell populations in mouse submandibular gland after X-irradiation. Methods: Submandibular glands of male C57BL/6 mice were locally X-irradiated with single dose irradiation with 7, 5 Gy. Tissue were processed for paraffin embedding at 1, 3, 6 and 10 days after X-irradiation. The apoptosis and proliferation were investigated using TUNEL method and immunohistochemistry for proliferation cell nuclear antigen (PCNA) in different gland compartments: acini, intercalated ducts and granular convoluted tubes. Results: At day 1 after irradiation, a significant decrease in PCNA-positive cells was noticed in different compartment of irradiated glands when compared to no irradiated glands (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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