| Autor: |
Berberović, Edina, Ivanišević, Marina, Horvatiček, Marina, Delaš, Ivančica, Djelmiš, Josip |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
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| Popis: |
Introduction. The essential long-chain polyunsaturated fatty acid, arachidonic and docosahexaenoic acid are important for fetal growth and development. Fetal concentration of AA and DHA depends on the mother’s concentration, i.e. on their ingestion by food. Previous studies showed that type 1 diabetes is associated with low AA and DHA levels of pregnant women. The aims of the study were: 1. To determine total FAs concentration in the maternal vein serum and in the umbilical vein serum in pregnant women suffering from T1DM and Control group ; 2. To determine and analyze AA and DHA concentrations and percentages (%) in relation to the total FAs concentration in the mother’s vein serum and the umbilical vein serum in pregnant women suffering from T1DM compared to the Control group. Methods. The study included 63 pregnant women, 32 suffering from T1DM and 31 from Control group. Samples of a mother’s vein blood and the umbilical vein blood were taken immediately after the birth. The total lipid extraction was carried out by the mixture of chloroform: methanol solvent: a method of increasing polarity according to Folch. Heptadecanoic acid was used as internal standard in order to determine the exact proportion of total lipids. Fatty acids from lipid extracts were converted to methyl esters by transesterification with methanolic HCl (ref: ISO standard) and their composition analyzed by GC-MS on Varian 3400 (Varian, USA) equipped with Saturn II ion trap mass spectrometer operating in the electron impact (EI) mode. Results. There was statistically significant difference in the total FAs concentration in the maternal vein serum and the umbilical vein serum between the two groups (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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