| Autor: |
Domijan, Ana-Marija, Duka, Ivan, Friščić, Maja, Maleš, Željan, Mitić, Božena, Hruševar, Dario |
| Přispěvatelé: |
Vuković, Nina, Šegota, Vedran |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Popis: |
The aim of this study was to examine the impact of Himalayan balsam (Impatiens glandulifera Royle, Balsaminaceae) extract on parameters of oxidative stress in white mustard (Sinapis alba L., Brassicaceae) and radish (Raphanus sativus L., Brassicaceae) seedlings. The seeds of mustard or radish (n = 25) were placed in Petri dishes on filter paper impregnated with I. glandulifera (Čučerje, Croatia) leaf methanolic extract in doses 1.5, 3 or 6 mL (in three replicates) and germinated in controlled environment for 3 days. In the experiments, negative (seeds exposed to distilled water) and positive controls (seeds exposed to 0.02 M CuSO4) were included. After germination, the seedlings were weighed, and plant tissue homogenate prepared. In supernatants of plant tissue homogenates polyphenols were assessed with Folin-Ciocalteu's reagent, anthocyanins by use of pH differential method, glutathione (GSH) with Ellman's reagent and malondialdehyde by TBA assay, spectrophotometrically. The difference among groups was tested by one-way ANOVA followed by Dunnett's test (p < 0.05). After 3-days of exposure to I. glandulifera extract in dose of 1.5 mL the reduction of fresh weight of mustard seedlings was observed, while even the highest dose (6 mL) of the extract had no effect on radish seedlings fresh weight. In mustard seedlings, 3- days of exposure to the highest dose of I. glandulifera extract (6 mL) reduced the levels of polyphenols and anthocyanins, and increased the level of GSH. The level of oxidative stress parameters in radish seedlings was not affected by 3-days of exposure to I. glandulifera extract. Reduction in the levels of polyphenols and anthocyanins and increase in the level of GSH indicate that extract of I. glandulifera induced oxidative stress in S. alba seedlings. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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