| Autor: |
Ivančić Šantek, Mirela, Mušak, Lucija, Petravić Tominac, Vlatka, Beluhan, Sunčica, Šantek, Božidar |
| Přispěvatelé: |
Radojčić Redovniković, Ivana, Radošević, Kristina, Jakovljević, Tamara, Stojaković, Renata, Gaurina Srček, Višnja, Erdec Hendrih, Dina |
| Jazyk: |
chorvatština |
| Rok vydání: |
2016 |
| Předmět: |
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| Popis: |
Lignocellulosic biomass have been studied extensively as renewable carbon source for production of second generation of biofuels such as bioethanol and biodiesel. In order to determine the content of unfermented carbon source as well as overall efficiency of the process it is necessary to determine the content of lignocellulosic carbohydrates. Therefore, a simple and reliable method for the analysis of major lignocellulosic components (glucan, xylan and lignin) was developed. Composition of three abundant lignocellulosic feedstocks in Croatia, corn cobs, corn husks and hay, was analysed. Firstly, lignocellulosic biomass was subjected to sulphuric acid hydrolysis prior to monosaccharide determination. The acid hydrolysis was optimized to give the maximal constituent monosaccharides. A high-performance liquid chromatography (HPLC)-based method using refractive (RI) was applied to determine concentration of monosaccharides in purified lignocellulosic hydrolysate. Monosaccharides were separated by HPLC using two different HPLC columns, i.e. Rezex RPM Monosaccharide Pb2+ (Phenomenex) and Supelcogel C610H (Sigma-Aldrich). Glucan and xylane content was calculated from the HPLC measured cellulose (glucose) and hemicellulose (xylose, galactose, mannose, arabinose) derived sugars, respectively. Furthermore, the content of acid soluble and acid insoluble lignin in lignocellulosic hydrolysate biomass was also determined. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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