| Autor: |
Berthelot, Françoise, Martinat-Botté, Françoise, Perreau, Christine, Terqui, Michel |
| Zdroj: |
Reproduction Nutrition Development; 2001, Vol. 41 Issue: 3 p267-272, 6p |
| Abstrakt: |
In swine, five to six days post-insemination, morulae and blastocysts are collected together after uterine flushing. The purpose of this study was to vitrify zona pellucida-intact morulae with Open Pulled Straw (OPS) technology and obtain piglets after transfer. Morulae (200) were vitrified after a two-step equilibration in ethylene glycol, dimethyl sulfoxide and sucrose in Hepes-buffered TCM199 + 20% NBCS medium (TCM). 2-6 morulae were loaded into OPS and plunged into liquid nitrogen. At embryo warming, a three-step dilution with decreasing concentrations of sucrose was applied. In each of 10 recipients, 20 morulae were transferred surgically. Day 25, gestation rate and the farrowing rate were 80% and 70% , respectively. The pregnant recipients farrowed from 1 to 8 piglets and the survival of total transferred embryos was 13% . Although survival rates are still compromised, OPS technology is therefore appropriate to cryopreserve porcine morulae with intact zona pellucida. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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